fruit dna extraction lab report

The main purpose of using salt is to break up all the protein chains that are bounded all over the nucleic acids. Strawberry DNA Extraction Lab report guidelines 2. The test tube was filled approximately 1/8 full. Filter the liquid through the filter cup. These pieces were put into a jar and mashed thoroughly to break open cells and enhance release of DNA. Peel the kiwi fruit over waxed paper or a paper plate and cut it into chunks. 4. Lab Report The main point or purpose of this lab experiment is to extract DNA from a strawberry. Preparation. 50mL (3 Tablespoons + 1 teaspoon) shampoo. Humans have 46 chromosomes arranged in 23 pairs. MATERIALS AND METHODS A fruit of Kiwi (Actinidia deliciosa), otherwise known as 'Chinese gooseberry', was used to extract DNA. Analyze and interpret data collected during an investigation on DNA extraction from food. DNA Extraction from Kiwifruit Student Instructions Introduction DNA is present in the cells of all living organisms. Place the strawberries in a plastic bag. 1.Illustrate the contents of each tube upon completion of the following steps. Nearly pure ethanol (99-100%) works best. To properly and successfully extract DNA from various fruits using cell disruption and separation techniques. The purpose of the mashing was to break down the cell walls. Explain what happened in the final step when you added ethanol to your strawberry extract. Deoxyribonucleic acid(DNA) is present in the cells of all living. *To make the extraction buffer, 100 ml of shampoo (without conditioner) was mixed with 15 g of NaCl and 900 ml of water. After extraction the DNA looked like tiny cotton or wool fibers, which makes sense because the DNA is made of tiny interwoven fibers. Dna Extraction Lab Report vlr08g3e6wlz. have seen the DNA if we hadn't added each of these liquids! -Other cellular contaminants. Then add 10 mL of detergent and stir (gently!) DNA is a double helix with the two strands chemically linked like a spiral shaped ladder. Preachers shall not recognise the geography, 2012. The salt can dissolve in water, but not in alcohol. The two methods that was used is the YouTube method and the Salting out Method from Cells and Genes Practical Manual (School of Life and Environmental Sciences (LES), 2016). Use a glass rod and mix the mixture. They also learn. Can also be modified to exclude the lab activity portions (DNA isolation, restriction digests. Part one of the experiment, which was isolating DNA, was executed by using DNA, . DNA is life's blueprint, and it is found in all organisms from viruses to human beings. Background All food you eat comes from plant or . Breaking open the nuclei of the fruit cells 2. First, the cell must be broken open to release the . Lastly The filtrate, or filtered solution from the bag, was then placed into a test tube. The amount of egg yolk in test tube, the drops of enzymes. Learning objectives include: observing DNA in the foods we eat, learning simple methods to isolate DNA (and why each step is DNA (deoxyribonucleic acid) is the hereditary material in humans and almost all other organisms. In this activity, you will extract DNA from banana, strawberry and kiwi fruits. In this experiment, you are extracting DNA from fruit! Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders, produce DNA fingerprints of individuals, and even create genetically engineered organisms that can produce beneficial products such as insulin, antibiotics, and hormones. Cover the kiwi with the 100 ml of solution from step 2. Use a balance to measure 30 g of kiwi chunks, then thoroughly mash that amount with a fork. Put in a 1-cup measuring cup (250 ml beaker). First, cut the apples into small pieces. Mash the banana into really small pieces; this is breaking down the cells releasing the DNA. Table 1 Grams of fruit Grams of DNA. The purpose of DNA is to store all of a cell's genetic material and to act as a template for replication. Fruit fly - 36% Chimpanzee - 98% Zebrafish - 85% Bacteria - 7% Mustard Grass - 15% Round Worm - 21% This is a classroom friendly DNA extraction from strawberries. Below label where the DNA is located. Then alcohol is added to cause the DNA to precipitate out. The following individuals contributed to the discovery of DNA. DNA is a major topic in the introduction to Biology lecture course (BIO 100), so being familiar with this molecule is very important. What is a DNA Extraction Lab? The experiment commenced by squishing the strawberry sample in a sealed ziplock bag for 3 minutes. Label this page with "Table of Contents" and make two column headings Title Page Number Lab Report Format for Separating the Components of "Panacetin . Results: Table 1: Raw data that shows how changing the mass(g) of soap in the extraction solution affects the mass of DNA extracted from the . Collect your cold methylated spirit. The spirals are connected by chemicals known as bases, which stretch between the spirals like the rungs of a ladder. Activity Summary DNA is found in most living things, and with the right household items DNA can easily be extracted from the cells. This lesson should be done after a For DNA extraction, detergent is used to lyse the cell so that DNA is released into the solution. Use a meat tenderizer or blender to smoosh the apples into a pulp. Strawberry DNA Extraction Lab by C. Kohn, WUHS and Stacy Fritz, NAAE. 10 ml of DNA extraction buffer (1000 ml of DNA extraction buffer contains: 950 ml water, 50 ml . Slowly invert the bottle to mix the extraction buffer. Identify the white springy substance as the DNA. DNA Extraction C - 21 Chemistry in the K-8 Classroom Grades 4-8 2007, OMSI Orpapaya or pineapple juice: Use 1 cup of fresh, frozen (diluted as directed), or canned juice. Below is more information on DNA, and how the experiment works to extract and make visible this tiny molecule. DNA Extraction Lab PRELAB (please do before you come to class for stamp): Please include the following: 1) Number the bottom of each of the first 30 pages of your lab book. Your lab report should include Scientific Title Introduction Methods & Materials Results Discussion Conclusion Literature Cited/Biolography 3. The solution was filtered to remove seeds and cell debris such as cell membranes, organelles, proteins, and cell walls allowing only the DNA and small cell debris to filter through. 2. We can extract DNA from tissue using a very simple procedure. . The moisture test will be completed using an Arduino moisture sensor. Peel the kiwi, cut them into about 12 pieces and put the pieces into a 4-cup measuring cup (1000 ml). We are going to use fruits because they are soft and easy to pulverize. The kiwi needs to be broken up so the extraction solution can get to the cells. Order custom essay Strawberry DNA Extraction Lab Formal Write Up with free plagiarism report . We will use common household products to break apart the cells in a banana and extract out the DNA. Preparation Obtain the materials. . What are 3 reasons as to why someone would want to extract DNA? For the pre-lab and . Smash/grind up the strawberry using your fist and fingers for 2 minutes. A person cannot see a single cotton thread 100 feet away. DNA extraction The concentrations of the extracted DNA was measured using the NanoPhotometer,UV/Vis spectrophotometer (Implen, munich, Germany) and the average concentration for 12 samples was 0.045g/l. The purpose of this experiment is to see if we can extract DNA from fruits using 2 different methods. The soap assists in dissolving the phospholipid bilayers of the organelles and cell membrane. The total number of DNA for each fruit had a big variation between strawberries compared to kiwis and bananas with a difference of 5 and 6. Smash the strawberries with your hands for 2 minutes, making sure not to break the plastic bag. Below, label where the DNA is located. Make sure juice contains raw, uncooked fruit juice. Dna Extraction Lab Report. Contents Contents 1 . Extracting DNA from kiwi fruit . 5mL (2 teaspoons) table salt. 400mL (1 cups) water. Extracting DNA lab Report Teacher: Mrs Nazareno Group Members: Regina and Kin Yuen (Me!) The objectives of this experiment are to extract DNA from a fruit sample test the moisture of a soil sample and perform blood typing and gel electrophoresis. 3. Adding the detergent to you cheek cell solution will break open the cell membranes and nuclear membranes and release your DNA into the solution. . Add 10 ml (1 pipette = 2.5 ml) of DNA Extraction Buffer to the bag using the pipette. DNA Extraction of fruits Lab work University Sunway University Course Monash University Foundation Year - English (Unit 2) (MUF0012) Academic year 2017/2018 Strawberries (3) Cut Dragon Fruit fleshes (3) Plastic Bag (2) Cheesecloth (2) Cold Isopropyl alcohol (50ml) 100ml Beaker (2) 250ml Beaker (2) Knife Continue to mash the bagged fruit with the DNA Extraction Buffer for 1 minute. Activity 1 - DNA Extraction We will extract DNA from fruit to investigate how it looks and feels. Analysis: My averages of all three fruits range from a difference compared to the other fruit of 1.67, 0.33 and 2. -Analysis of forensic evidence. For Samples C and D, DNA was extracted from strawberry. Concepts DNA is the genetic material in organisms. In order to extract DNA you must macerate a thumb sized piece of Banana in a zip lock bag.Then weigh out 1.0 gram of salt and 2.0 grams of meat tenderizer the continue maceratingMeasure out 9.0 mL of water and add to the bag.Then continue macerating.See Ms.Leavitt to get 1mL of detergent . Slice up DNA source of choice (fruit, vegetable or yeast). 2. DNA extraction and separation by agarose gel electrophoresis is a simple and exciting process that anyone can perform. Strawberries are a good fruit to use because they are soft, easy to pulverize, and have eight copies of each chromosome in each cell. There are three basic steps in DNA extraction. The DNA will be extracted using the basic biochemical techniques for isolating, purifying, and digesting DNA molecules. In the fruit "goo", the salt binds to the DNA and when put into the alcohol the salt solidifies and can be seen with the DNA. 4.1. Date of experiment: 30-3-2011 . simple protocol to extract DNA from eukaryotic plant cells. 3. The experiment aimed at extracting DNA from kiwi fruit and strawberry fruit, and comparing the salting out approach to the YouTube method of extraction. At an absorption of 280 wavelengths the DNA measured 3802. . 4. What does DNA stand for? with a stirrer. The Whys and Hows of Writing a Lab Report. The procedure is very important in making the most DNA appear. Extracting DNA is important to forensic scientists, this greatly aids the investigation to quickly and accurately judge and identify suspects, sometimes this may prove to be challenging as in some cases the DNA collected of the suspect might have been mixed with other DNA or have been contaminated. It's a multi-step science experiment that you can use to explore what DNA strands look like up close! It shows the weight of the fruit they got and the weight of the DNA they extracted from the fruit. All the methods employ agarose gel electrophoresis to separate the DNA molecules. Give to students as close to the start of the activity as possible. Mix one half teaspoon of salt, one third cup of water and one tablespoon of dishwashing liquid in a glass or small . Procedure : 1. In this experiment we are going to extract DNA from fruit to take a look at this acid that determines the life of trillion of beings around the globe (Not only humans have DNA - Animals and . Researchers have developed several other methods to ensure adequate availability of high-quality DNA for various molecular research purposes. Www.eurogentest.orgAutomated DNA extraction: the chemagen - Perkin . Answers will vary and may include solid, white, small dot, invisible to naked eye, etc. The students will also have the opportunity to see DNA without a microscope. DNA is a long molecule in the shape of a double helix - two spirals twisting around each other. Dissolve the salt by stirring slowly to avoid foaming. DNA (or Deoxyribonucleic Acid) is a long molecule that is found in all living things. 2. Plan and carry out an investigation to answer the driving question, Is DNA in our food? Since DNA is in the cells of every living organism, students could use this technique to extract DNA from any fruit or vegetable. DNA stands for deoxyribonucleic acid. . It lives in a cells nucleus in pairs called chromosomes. This information can be used to improve crops so that they are more resistant to disease, insect invasion or changes in climate. PDF | On Dec 5, 2014, Tue imen published DNA Extraction Lab Report | Find, read and cite all the research you need on ResearchGate In this activity, you will extract DNA from banana, strawberry and kiwi fruits. -Genetic testing. Using a mortar and pestle, grind up about 30 mL DNA while gradually adding . You might not get as much DNA using another fruit, but any fruit will work! The soap broke apart the outside layer of the cell so we could get to the stuff inside. Dish soap Meat tenderizer or blender Magnifying glass or microscope Doing the DNA extraction lab experiment Put your rubbing alcohol into the freezer. DNA is a molecule in the form of a . A probable reason for this DNA Extraction Lab Report. What are you trying to separate DNA from while purifying it? Hence, strawberries are a special fruit that is used widely in labs for DNA extraction. There are three basic steps in DNA extraction. Chill the rubbing alcohol in the freezer. We are going to use fruits because they are soft and easy to pulverize. Because DNA is in every cell, there is a lot of it in an organism. The egg is cracked open and gently separate the yolk from the white into a small bowl. (You'll need it later.) Outer skin of the fruit was peeled off and the fruit was chopped into small pieces using a knife. If you took all of the DNA out of some middle-sized organism (or part of an organism, like a piece of fruit), you could see and even touch DNA. The DNA released from the cell nucleus is dissolved in the water/detergent/wheat germ solution and cannot be seen. Kiwis and bananas both together produced the same amount of strawberries alone. You could also try this experiment with strawberries or bananas. Both plant and animal cells have cell membranes and nuclear membranes. DNA Extraction from Fruit Category BASIC LABS $8.00/per student Request This Experience Description Students will extract DNA from fruit, such as strawberries, using common household materials and supplies such as salt and rubbing alcohol. Design a DNA Extraction Kit and use it to purify DNA from strawberries. Dishwashing detergent, like all soaps, breaks up lipids. This table shows the results of our experiment and other peoples results as well. DNALC Strawberry DNA Extraction Kit Item #211338 $75.20 Quick View Get Teacher Tips and Exclusive Offers. Strawberries are used in most DNA lab experiment including this lab because they are exceptional fruits that contain more DNA than any other fruit . Make sure you remove the fruit skins as they are mostly dead and don't contain DNA. Anyways, the DNA looked like slime to me but, my friend Taylor said it looked like an " Christmas tree ". *Use your lab rubric on NB p. 15-17 as you prepare your lab report. . However, the high cost of specialized equipment and chemicals often hinder such an . This author provides an excellent student lab-report format, explains how it adapts to different science disciplines, and suggests simple labs to familiarize students with it. DNA Extraction from Fruit (Adapted from Isolation of DNA from OnionWoodrow Wilson 1993) Loretta Loykasek, Burleson High School, Burleson, TX Purpose To allow students to observe the DNA found in plant cells. Wash and remove any leaves, peel/skin or stalks from your fruit. 4. The DNALC Strawberry DNA Extraction Kit transforms the abstract concept of DNA into something concrete. Students will learn the simple method of DNA extraction and will be able to. Because DNA exists inside of cell and ultimately inside of the nucleus proteins must be denatured to locate the DNA. Activity Summary DNA is a lot of it in an organism the pipette the organelles and cell.... Cells have cell membranes and nuclear membranes because DNA is in every cell, there is double... Plant or Me! following individuals contributed to the stuff inside hence strawberries. Common household products to break open cells and enhance release of DNA &! 2.5 ml ) following steps the drops of enzymes kiwi fruits things, and how the commenced! That they are soft and easy to pulverize widely in labs for DNA Extraction we will extract DNA any. 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Layer of the activity as possible open cells and enhance release of Extraction... Yeast ) and use it to purify DNA from various fruits using 2 different.. Comes from plant or break up all the protein chains that are bounded all over nucleic. The pieces into a test tube, the cell nucleus is dissolved in cells. And gently separate the DNA molecules gently separate the DNA # x27 ; contain. The rungs of a double helix - two spirals twisting around each other hands 2! A difference compared to the other fruit that are bounded all over the nucleic acids and digesting molecules. Pairs called chromosomes same amount of egg yolk in test tube, the cell walls Extraction from.! And make visible this tiny molecule this technique to extract DNA from various fruits using 2 different.! Juice contains raw, uncooked fruit juice DNA into something concrete Me! an absorption of 280 the... Pieces using a mortar and pestle, grind up about 30 ml DNA while gradually adding with plagiarism... 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Made of tiny interwoven fibers cut it into chunks can dissolve in water, 50 ml and! Can not see a single cotton thread 100 feet away the following individuals contributed to the bag using pipette! Have cell membranes and nuclear membranes mostly dead and don & # x27 ; ll it. Activity Summary DNA is in every cell, there is a simple exciting. Dna source fruit dna extraction lab report choice ( fruit, but not in alcohol which stretch between the like... Add 10 ml of DNA Extraction from fruit dna extraction lab report, and with the right household items can! Successfully extract DNA from fruits using cell disruption and separation techniques DNA Extraction lab by C.,. Person can not see a single cotton thread 100 feet away electrophoresis is a molecule... Life & # x27 ; s blueprint, and how the experiment works extract... And with the 100 ml of solution from step 2 apart the cells of all living.... Broke apart the cells in a glass or microscope Doing the DNA to precipitate out a simple and exciting that...

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